JMDplots vignettes

Hyperosmotic Gene Expression

This vignette from the R package JMDplots version 1.2.19-9 shows chemical metrics for proteins coded by genes that are differentially expressed in hyperosmotic compared to control conditions. The analysis is described in more detail in a paper (Dick et al., 2020). Abbreviations:

datasets <- pdat_osmotic_gene(2020)

Differences are calculated as (median value for proteins coded by up-regulated genes) - (median value for proteins coded by down-regulated genes). Dashed lines enclose the 50% credible region for highest probability density.

In the table, values of ΔZC, ΔnH2O, and ΔGRAVY are multiplied by 1000, values of ΔpI and ΔMW are multiplied by 100, and negative values are shown in bold. Abbreviations:

set reference (description) ndown nup ΔZC ΔnH2O ΔpI ΔGRAVY ΔnAA ΔMW
a KSA+02 (Synechocystis sp. PCC 6803 in 0.5 M NaCl vs control) 36 62 8 15 28 62 33 -106
b KSA+02 (Synechocystis sp. PCC 6803 in 0.5 M sorbitol vs control) 32 45 -3 -6 25 -16 -2 52
c WJ02 (Escherichia coli in 0.4 M NaCl vs control) 102 41 9 1 8 -8 -32 15
d HZP+05 (Yersinia pestis in 0.5 M NaCl vs control) 13 103 -6 -24 -38 -11 55 144
e HZP+05 (Yersinia pestis in 0.5 M sorbitol vs control) 40 103 11 -44 -36 -65 181 123
f LGW+05 (Shewanella oneidensis MR-1 in 0.5 vs 0.1 M NaCl) 563 541 2 15 -6 7 -19 -46
g SLA+05 (Synechocystis sp. PCC 6803 in 0.5 M sorbitol vs control) 27 53 19 26 -62 -74 103 92
h GCP08 (Escherichia coli in 0.5 M vs 0 M NaCl at 30 deg C) 307 40 11 5 -35 -64 168 -155
i GCP08 (Escherichia coli in 0.5 M vs 0 M NaCl at 43 deg C) 89 144 1 -7 -12 2 96 -40
j SBB+09 (Escherichia coli in ~2.7 Os/kg NaCl vs control) 35 26 -34 25 -47 150 -115 5
k SBB+09 (Escherichia coli in ~2.7 Os/kg sucrose vs control) 28 37 -32 28 26 151 72 14
l HMO+10 (Bacillus subtilis in 6% w/v NaCl vs control) 417 459 -2 1 30 -8 -33 73
m BBWB12 (Listeria monocytogenes strain H7858 in 6% NaCl at 2.5% lag-phase duration) 18 55 16 49 -51 -74 -18 -235
n BBWB12 (Listeria monocytogenes strain H7858 in 6% NaCl at 5% lag-phase duration) 85 134 7 -15 -64 -10 -70 -50
o BBWB12 (Listeria monocytogenes strain H7858 in 6% NaCl at 10% lag-phase duration) 136 156 7 -18 -65 24 -65 -104
p BBWB12 (Listeria monocytogenes strain H7858 in 6% NaCl at 20% lag-phase duration) 58 44 2 8 -42 129 39 -268
q LB12 (Synechococcus sp. strain PCC 7002 in 1.5 M NaCl vs control) 311 646 6 11 -61 41 2 -150
r QHT+13 (Synechocystis sp. PCC 6803 Gene in 4% w/v vs 0% added NaCl for 24 h) 50 48 13 56 111 39 74 -194
s QHT+13 (Synechocystis sp. PCC 6803 Gene in 4% w/v vs 0% added NaCl for 48 h) 50 50 -7 -49 76 27 -12 56
t QHT+13 (Synechocystis sp. PCC 6803 Gene in 4% w/v vs 0% added NaCl for 72 h) 50 49 -20 5 152 156 68 -30
u WGB+13 (Escherichia coli in 0.3 M NaCl vs control) 525 524 12 -46 66 55 -34 65
v WGB+13 (Escherichia coli in 0.6 M urea vs control) 337 370 -1 5 152 -36 -74 130
w ADW+14 (Bifidobacterium longum BBMN68 Gene with vs without 0.75 g/l ox bile) 160 76 12 -51 -30 -169 -46 202
x KKG+14 (Escherichia coli Gene in NaCl (0.967 aw) vs control for 30 min) 15 164 3 26 -65 122 7 -58
y KKG+14 (Escherichia coli Gene in NaCl (0.967 aw) vs control for 80 min) 409 727 11 -16 19 -54 -35 83
z KKG+14 (Escherichia coli Gene in NaCl (0.967 aw) vs control for 310 min) 319 571 7 -21 -8 -71 -38 74
A KSM+14 (Bacillus subtilis in 1.2 M NaCl vs control) 687 642 -6 -7 11 63 4 6
B KSM+14 (Bacillus subtilis with vs without glycine betaine in 1.2 M NaCl) 311 559 8 10 -21 -16 4 -41
C MGM+14 (Escherichia coli in 3.5 vs 2% NaCl (with glycine betaine)) 169 64 7 -13 25 -5 11 15
D MGM+14 (Escherichia coli in 4.5 vs 2% NaCl (with glycine betaine)) 740 579 -9 -30 60 167 56 -65
E MGM+14 (Escherichia coli in 5 vs 2% NaCl (with glycine betaine)) 43 82 8 -14 34 -99 36 68
F MGM+14 (Escherichia coli in 5.5 vs 2% NaCl (with glycine betaine)) 79 84 15 -11 30 -16 -18 -22
G SLM+14 (Enterococcus faecalis in 6.5% NaCl vs control at 5 min) 113 69 -6 -17 74 79 82 -4
H SLM+14 (Enterococcus faecalis in 6.5% NaCl vs control at 30 min) 125 175 -4 -27 48 -2 -39 166
I SLM+14 (Enterococcus faecalis in 6.5% NaCl vs control at 60 min) 170 172 3 -57 -152 47 67 16
J FRH+15 (Salmonella enterica in NaCl vs control for 1h h) 113 60 16 81 -185 55 72 -357
K FRH+15 (Salmonella enterica in NaCl vs control for 6h h) 255 149 7 25 -7 -11 62 -133
L FRH+15 (Salmonella enterica in NaCl vs control for 24h h) 163 60 0 -48 35 -66 -51 125
M FRH+15 (Salmonella enterica in KCl vs control for 1h h) 92 45 13 59 -214 19 105 -343
N FRH+15 (Salmonella enterica in KCl vs control for 6h h) 349 264 3 3 -10 -80 -18 40
O FRH+15 (Salmonella enterica in KCl vs control for 24h h) 403 182 15 -21 -12 -53 66 -13
P FRH+15 (Salmonella enterica in glycerol vs control for 1h h) 300 333 -8 12 11 6 -18 10
Q FRH+15 (Salmonella enterica in glycerol vs control for 6h h) 261 132 -18 -35 46 60 -50 109
R FRH+15 (Salmonella enterica in glycerol vs control for 24h h) 164 67 0 -52 27 -35 -41 90
S KLB+15 (Caulobacter crescentus Gene in 40/50 mM NaCl vs M2 minimal salts medium) 209 142 9 -45 47 109 -8 -125
T KLB+15 (Caulobacter crescentus Gene in 200 mM sucrose vs M2 minimal salts medium) 105 96 27 -65 -70 73 42 -100
U HLL17 (Methylocystis sp. strain SC2 in 0.75% NaCl vs control at 45min) 105 46 -7 42 170 179 -11 -187
V HLL17 (Methylocystis sp. strain SC2 in 0.75% NaCl vs control at 14h) 22 75 22 -34 -195 154 16 -39
W MWZ+18 (Lactobacillus paracasei L9 with vs without 0.13% ox bile) 42 56 2 -35 -4 -57 -22 12

Data Sources

a. b. Tables 1–2 of Kanesaki et al. (2002). c. Table 1 of Weber & Jung (2002). d. e. Table 3 of Han et al. (2005). f. Table 1S of Liu et al. (2005). g. Tables 2–3 of Shapiguzov et al. (2005). h. i. Dataset.txt of Gunasekera, Csonka & Paliy (2008). j. k. Table 3 of Shabala et al. (2009). l. Table S2 (sheet “S2_Table_3”) of Hahne et al. (2010). m. n. o. p. Table S5 of Bergholz et al. (2012), filtered to include genes with log2 fold change > 1 or < -1. q. Table S2 of Ludwig & Bryant (2012), filtered to include genes with p-value < 0.05 and fold change > 2 or < 0.5. r. s. t. Supplementary Tables S2A–S2F of Qiao et al. (2013). u. v. Dataset.txt of Withman et al. (2013), filtered to include proteins with log fold change (column “N vs K Wmean [M]” for NaCl and “U vs K Wmean [M]” for urea) > 1 or < 1 and p-value less than 0.05. w. Table S2 of An et al. (2014) x. y. z. Table S2 of Kocharunchitt et al. (2014) A. B. Tables S5–S6 of Kohlstedt et al. (2014). C. D. E. F. Supplemental Table S1 of Metris et al. (2014), filtered to include genes with differences of Normalized ln(cDNA/gDNA) > 1 or < 1 between 2 % and higher NaCl concentration. G. H. I. Table S2 of Solheim et al. (2014). J. K. L. M. N. O. P. Q. R. Tables S1–S3 of Finn et al. (2015). S. T. Table S2 of Kohler et al. (2015). U. V. Tables S5–S8 of Han, Link & Liesack (2017). W. Table S6 of Ma et al. (2018).

References

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